Is PolyA RNA-seq equal to 3'Tagged RNA-seq?

Question

[u3005992]

Hi

Is PolyA RNA-seq equal to 3'Tagged RNA-seq?

Are those two the same thing?

James

[GouthamAtla]

No

Answer 1

[ATpoint]

No, polyA-based RNA-seq only means that transcripts were extracted that are polyadenylated. These can then be used for either full-length or 3'-tagged RNA-seq. 3'-tagged only means that the 3'-part of the transcript was enriched furing library prep in contrast to the entire fragmentated transcript/cDNA.