Do tools doing clonal evolution analysis work on unmatched samples too

Question

[zizigolu]

Hi

I have biopsies of pre chemotherapy patients and biopsies of post chemotherapy of different patients I mean pre and post treatment biopsies are not the same patients I want to look at the clonal evolution of tumour following chemotherapy hopefully to find resistant clones or anything meaningfully happen by treatment

Can I still use tools like scieclone software?

I have asked this from the developer but no answer yet

Please you may share your idea with me

Thanks

Answer 1

[igor]

If they are different patients, then they are different clones. Clonal analysis is used to track the same clones across different time points.

[zizigolu]

Yes they are different patients. So do you know a way to find the resistant clones from unmatched patients?

[igor]

Then you can compare specific variants. For example, mutation X is present in one group, but not the other.