Question: Minimum # markers per chromosome/scaffold for imputation with Beagle 5.1
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gravatar for dario.galanti92
6 months ago by
dario.galanti920 wrote:

I have a multisample vcf file from a study species with a pretty fragmented reference genome. I already applied the basic filtering steps to my vcf using vcftools. 1- Sample filtering: filter out low coverage samples 2- Site and genotype filtering with the following vcftools parameters: --minDP 3 --max-missing 0.8 --maf 0.05 I am now trying to run imputation of missing genotypes using BEAGLE 5.1 and it is running smoothly until it reaches a small scaffold that only has 1 position in the vcf. So clearly I need to erase scaffolds with too few positions from my vcf file, but here is my question: how many positions per scaffold is too few? I tried to look into the BEAGLE documentation but I couldn't find an answer, hopefully someone here can help?!

beagle snp imputation vcf • 211 views
ADD COMMENTlink modified 6 months ago • written 6 months ago by dario.galanti920
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gravatar for dario.galanti92
6 months ago by
dario.galanti920 wrote:

Solved it! I removed all scaffolds harbouring less than 3 SNP in my vcf file and it worked smoothly! Good luck to anyone else having the same problem!

ADD COMMENTlink written 6 months ago by dario.galanti920
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