when i run the following:
~ $picard FastqToSam \
F1=usr/local/Cellar/picard-tools/2.22.3/bin/untrimmed_reads.fastq \
O=unaligned_reads.bam \
SM=sample001 \
RG=r0013
I get
-bash: F1=usr/local/Cellar/picard-tools/2.22.3/bin/untrimmed_reads.fastq: No such file or directory
Running
~ $picard FastqToSam -h
gets me the help screen.
changing my directory and running
java -jar picard.jar FastqToSam \
F1=usr/local/Cellar/picard-tools/2.22.3/bin/untrimmed_reads.fastq \
O=unaligned_reads.bam \
SM=sample001 \
RG=r0013
Also, doesn't work.
What am I doing wrong? The file exists!
Figured it out. problem two was my issue. This is the first time I've ever messed with anything more then python scripts and I didn't realize how different usr is from user.