Hi biostars community! I am trying to download the original fastq paired-end read files from three biosamples of a bioproject from SRA. For example: https://trace.ncbi.nlm.nih.gov/Traces/sra/?run=SRR8581464
There are 4 original fastq files. Trying to download with SRA toolkit prefetch command "prefetch --type fastq SRR8581464" results in the following error:
"prefetch.2.10.5 err: error unexpected while resolving query within virtual file system module - failed to resolve accession 'SRR8581464' - The object is not available from your location. ( 406 )"
without the --type switch it downloads the sra file, which does not help me because the original files contain reads which I want to analyse separately (these are reads from DNA containing extracellular vesicles of different densities).
I think the original files can only be downloaded from amazon/google cloud, but I don't understand how to do so. The ncbi documentation only describes how to generate a cloud VM and install their toolkit there, which I don't want to. I just want those files on my machine… I am astonished how they managed to make something so simple as downloading files that complicated.
Thank you very much in advance for you help. Cheers! Stefan