I am very new to image analysis. I wish to find out if any one can help me with information about programmatic tools of any type that can be used to quantify microscopic images from histological stainings.
I have performed a Sirius red staining to stain for collagen in my tissues. With image J, I discover that the collagen content is exagerated by normal areas of high collagen staining (which also stain red) such as the areas of the airways, compared to pathological areas with high collagen content. this exageration is partly because I find it very difficult, almost impossible, to set a colour threshold to clearly distinguish between normally high collagen-content areas and pathologically high collagen-content areas. I want to be flexible enough to prevent this exageration from normal high collagen-content areas.
Thank you in advance for your kind help.