Hello people!
I ran MIRA and the process was stopped. I went to analyze your "log" file and found this:
Loading reads from /../Pedobacter/SRR1769012.fastq type fastq [0%] .... | .... [10%] .... | .... [20%] .... | .... [30%] .... | .. .. [40%] .... | .... [50%] .... | .... [60%] .... | .... [70%] .... | .... [80%] .... | .... [90%] .... | .... [100%] Looking at FASTQ type ... guessing FASTQ-33 (Sanger) Running quality values adaptation ... done. Killed Failure, wrapped MIRA process aborted.
In another execution - with other readings - MIRA performed perfectly and in the same "place" that in the other execution gave an error he displayed this message:
Loading reads from /.../MycobacteriumUlcerans/ERR73267.fastq type fastq [0%] .... | .... [10%] .... | .... [20%] .... | .... [30%] .... | .. .. [40%] .... | .... [50%] .... | .... [60%] .... | .... [70%] .... | .... [80%] .... | .... [90%] .... | .... [100%] Looking at FASTQ type ... guessing FASTQ-33 (Sanger) Running quality values adaptation ... done. Checking reads for trace data (loading qualities if needed): [0%] .... | .... [10%] .... | .... [20%] .... | .... [30%] .... | .. .. [40%] .... | .... [50%] .... | .... [60%] .... | .... [70%] .... | .... [80%] .... | .... [90%] .... | .... [100%] No SCF data present in any read, EdIt automatic contig editing for Sanger data is now switched off.
Can I conclude that what went wrong with the first assembly was MIRA's quality module?
