Training in Advanced Transcriptomics (RNA-Seq) Analysis
June 9-12,2020
Where?
National Institutes of Health(ONLINE)
9000 Rockville Pike
Building 60, Room 162
Bethesda, MD 20892, USA
Objectives
Apart from performing the routine differential expression analysis using two different suite of tools, this hands-on training will help participants learn advanced RNA-Seq analysis techniques and tools for detecting snps, fusion genes, allele specific expressions, circular RNAs, viral/bacterial sequence identification, alternative polyadenylation and transcriptional regulatory network analysis.
Simultaneous access to two screens is highly recommended for adequate learning experience. Examples include two laptops, one computer with two screens, one laptop and one tablet, etc.
Hands-on Skills/Tools Taught
- Differential expression analysis: HISAT2 tools
- Differential expression analysis: Trinity tools
- Fusion gene analysis: STAR-Fusion
- SNP detection: GATK
- Allele specific expression: WASP
- Circular RNA identification: circexplorer
- Alternative polyadenylation: DaPars
- Viral integration: VirTect
- Network analysis: Cytoscape
Highlights
- Participants will use a Graphic User Interface based Linux Desktop environment, specially configured for advanced RNA-Seq analysis in the Amazon Cloud
- Training provided by experienced active NIH researchers
- PDF of a cookbook style manual for all exercises
- Continuing Education Credits
For more information and registration, please visit the following page: Information and Registration
