Entering edit mode
3.9 years ago
tianshenbio
▴
170
I tried to create blast database for my fasta file:
>ban-mir-276
agcgagguauagaguuccuacguaauacaugccuguaggaacuucauaccgugcucu
>ban-mir-9a
ucuuugguuaucuagcuguaugaguacucagacaucauaaagcuagguuaccggaguua
>ban-bantam
cugguuuucauaaugauuugacagauuguuccauauucugagaucauugugaaagcugau
>ban-mir-279b
aagggguuuaaaucuaguagcacaguguucaccugugacuagaucuacacucauuga
using command:
makeblastdb -in hairpin_name.fa -dbtype nucl -out hairpin_name -parse_seqids
An error message pops up:
Building a new DB, current time: 05/15/2020 20:26:49
New DB name: /media/nus/AMLab_Ext1/Shen/miR_anno/rm_redundancy/hairpin_name
New DB title: hairpin_name.fa
Sequence type: Nucleotide
Keep MBits: T
Maximum file size: 1000000000B
No volumes were created.
BLAST Database creation error: 'ban-mir-276' is not a valid local ID
Em...But there is no such ? in my original file
Perhaps there is a character that is not visible. Edit that line and type it in and see if that fixes things.
Thank you so much. The problem is solved. Not sure how the invisible thing is introduced...