I think this is a basic question, but somehow I am really confused right now and it would be amazing if somebody could point me the right way.
I was wondering how it is possible to perform DGE for a single cancer patient. I thought, that if you use programs like DESeq2, you need biological replicates to perform the statistical analysis. And if you are only investigating one patient you do not have different replicates. If we take several samples from the same tumor, this would be considered technical replicates right? Is there a way to circumvent this problem, or is it just not possible, to do this for a single individual patient?
Any help is appreciated!
Thank you very much, Nick