Off topic:Repost: Quality of RNA Seq Data
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3.9 years ago

Hello All,

I am reposting the question for answer:

I am analyzing human RNA Seq data from a paper (PRJNA421274). I did FastQC on the RAW data and it showed high difference between %A and %T as shown in the link below

https://ibb.co/vX6kL95

Even after trimming it is the same.Is it unsusal and should i use this samples? Is there a way to reduce this difference? I am new to RNA Seq Analysis.Any help is appreciated!

Thank you in advance.
RNA-Seq Quality sequencing rna-seq • 296 views
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