MOTHUR "screen.seqs" command option
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17 months ago
dpc ▴ 210

Hi friends!!! When we use for "screen.seqs" command in order to remove bad sequences (before alignment) how the sequence length should we choose? I mean what should be the value for the "minlength" and "maxlength" options? How should we determine this?

For example, suppose, I'm analysing V4-V6 region which is around 550bp long. And my sequences shows like the attached image. Here, what "minlength" & "maxlength" value should I use?

please click here for the image

mothur 16s metagenome microbiome • 333 views
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