Differential gene expression analysis
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2.2 years ago
ATCG ▴ 370

I am performing differential gene expression using DESeq2 but will be subsetting transcripts based on a pre-determined criterion. Does this sub-setting violate any assumptions make by the statistical package?

RNA-Seq DESeq2 • 618 views
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Answered by the DESeq2 author: https://support.bioconductor.org/p/131401/

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2.2 years ago

If you use too small a subset, the assumptions underlying the normalization procedure may be violated.

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One assumption is, that the majority of genes are not differentially expressed.

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