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121,580 results • Page
1 of 2432
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Votes
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0
votes
1
reply
101
views
Annotation comparison files on NCBI of gene assembly
annotation
updated 23 hours ago by
GenoMax
152k • written 23 hours ago by
zirui
• 0
1
vote
1
reply
157
views
Kraken2/bracken with 16S sequencing downstream analysis
microbiome
QIIME2
DADA2
metagenomics
kraken2
updated 1 day ago by
andres.firrincieli
3.9k • written 1 day ago by
Isidro
• 0
0
votes
0
replies
100
views
News:
Online Course – LLM-based tools for R (28 & 31 October 2025)
LLMs
AI
R
1 day ago by
Physalia-courses
★ 2.6k
843
votes
170
replies
183k
views
112 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 11 weeks ago by
Biostar
3.6k • written 8.6 years ago by
Istvan Albert
102k
0
votes
1
reply
152
views
inferCNV on Visium 10X: lower residual expression in tumoral spots (heatmap interpretation)
inferCNV
CNV
Visium
score
updated 1 day ago by
GenoMax
152k • written 1 day ago by
Gabriele
• 0
2
votes
2
replies
7.2k
views
6 follow
Any bacterial databases that contain Gram staining information?
gram-positive
gram-negative
written 9.0 years ago by
archaeopteryx
• 0
0
votes
1
reply
154
views
Two config files for one Snakemake file
config
yaml
snakemake
updated 1 day ago by
Bastien Hervé
6.4k • written 1 day ago by
Xiaokang
▴ 80
8
votes
4
replies
315
views
Exome data analysis computing resources requirement query
exome
updated 1 day ago by
GenoMax
152k • written 2 days ago by
1769mkc
★ 1.3k
6
votes
5
replies
11k
views
TCGA MSI status
TCGA
clinical
MSI
updated 2 days ago by
GenoMax
152k • written 8.7 years ago by
jan
▴ 170
0
votes
1
reply
170
views
Summing the counts of duplicated genes in RNA seq data?
genes
Duplicated
updated 2 days ago by
GenoMax
152k • written 2 days ago by
toobavaraste
• 0
1
vote
0
replies
125
views
Assembling a derivative chromosome from ONT WGS data
ONT
assembly
Whole-Genome
2 days ago by
Noah
▴ 20
2
votes
5
replies
272
views
[VG sim, surject, giraffe] How to remove a sample from GFA/GBZ graph
vg
2 days ago by
saruman
• 0
0
votes
10
replies
463
views
Strandedness issues in STAR quantification
STAR
stranded
updated 1 day ago by
GenoMax
152k • written 2 days ago by
marek.gierlinski
• 0
0
votes
1
reply
172
views
naccess installation error
naccess
updated 2 days ago by
GenoMax
152k • written 2 days ago by
Angelo
• 0
1
vote
2
replies
837
views
Download Isoform expression data from tcga
cancer
transcript
gene
isoform
updated 2 days ago by
GenoMax
152k • written 2.8 years ago by
nourmahfel
▴ 10
6
votes
11
replies
3.3k
views
Visualization of RNA-seq gene expression only one gene of interest between 2 conditions
RNA-seq
updated 2 days ago by
rajdeepboral00
▴ 70 • written 21 months ago by
Chris
▴ 360
0
votes
1
reply
167
views
feature_ref files in upstream analyses related to Perturb-seq
perturb-seq
updated 2 days ago by
GenoMax
152k • written 2 days ago by
2443422660
• 0
1
vote
2
replies
210
views
Splitting chromosomes into contigs within BAM and GTF files
samtools
gtf
chromosome
annotation
bam
2 days ago by
MAB
• 0
5
votes
7
replies
481
views
Forum:
Theoretical Question: Is Sub-Angstrom Geometric Identity (RMSD < 0.1 Å) in Convergent Evolution Possible?
Kinesin
Biophysics
Biology
Molecular
Myosin
updated 2 days ago by
GenoMax
152k • written 3 days ago by
Alberto
▴ 10
0
votes
0
replies
136
views
News:
online course: An Introduction to Nanopore Direct RNA Sequencing (RNA004) - 27–30 October
DirectRNAseq
Nanopore
RNA004
RNAModifications
3 days ago by
Physalia-courses
★ 2.6k
1
vote
2
replies
213
views
Refflat file for Rattus norvegicus
file
RefFlat
Rattus
RNA-seq
norvegicus
updated 3 days ago by
GenoMax
152k • written 3 days ago by
AIMAR
▴ 50
3
votes
6
replies
409
views
Forum:
LogFC on geometric means vs on arithmetic means. Presto vs Seurat
Presto
Seurat
logFC
general
questions
updated 2 days ago by
ATpoint
88k • written 3 days ago by
e.r.zakiev
▴ 260
1
vote
1
reply
476
views
Question about anova-like test using Limma
makeContrasts
limma
updated 3 days ago by
Gordon Smyth
★ 8.2k • written 13 months ago by
Daniel
▴ 10
5
votes
3
replies
362
views
asking for optimized bash scripting
analysis
updated 2 days ago by
Joe
22k • written 3 days ago by
QX
▴ 80
0
votes
5
replies
358
views
Differential expression genes
SEQ
DGE
RNA
updated 2 days ago by
GenoMax
152k • written 3 days ago by
m90
▴ 30
2
votes
3
replies
385
views
Discrepancy Between Fragment Size Distribution and MACS3-Predicted Fragment Length in ChIP-seq Dataset
macs3
2 days ago by
christoph.neu
▴ 10
3
votes
4
replies
332
views
Plotting of genes using tpm matrix
normalization
TPM
boxplot
updated 2 days ago by
i.sudbery
21k • written 3 days ago by
rajdeepboral00
▴ 70
3
votes
2
replies
302
views
A question about model matrix and contrast
makeContrasts
limma
3 days ago by
jkim
▴ 210
0
votes
1
reply
410
views
Understanding MAG completeness and contig composition in metagenomic bins
data
shotgun
updated 3 days ago by
andres.firrincieli
3.9k • written 8 days ago by
shevch2009
▴ 20
2
votes
4
replies
378
views
Understanding NCBI vs ENA data
ENA
NCBI
SRA
updated 3 days ago by
GenoMax
152k • written 4 days ago by
wes
▴ 90
0
votes
2
replies
280
views
Job:
Assistance running population specific hapibd
ancestry
haplotype
IBD
updated 3 days ago by
cmdcolin
★ 4.3k • written 4 days ago by
CJ169
• 0
2
votes
1
reply
223
views
Empty result using enrichGO
clusterProfiler
updated 3 days ago by
GenoMax
152k • written 4 days ago by
Chris
▴ 360
2
votes
1
reply
5.8k
views
Convert ct file to dot bracket format
RNA
structure
updated 4 days ago by
zahraniasari.2023
• 0 • written 8.6 years ago by
abdullahrenk
▴ 30
0
votes
2
replies
1.1k
views
News:
Hands-On Workshop - Next-Generation Sequencing Data Analysis: A Practical Introduction (September 10-12, 2025 in Berlin, Germany)
Workshop
RNA-Seq
Illumina
VariantCalling
DNA-Seq
4 days ago by
ecSeq Bioinformatics
▴ 20
0
votes
0
replies
169
views
Is it possible to perform XP-EHH and iHS on targeted sequencing data without a .map file?
ngs
ihs
analysis
selection
sequencing
4 days ago by
slzr_
▴ 10
0
votes
1
reply
228
views
Discrepancy in RNA editing calls between STAR and Subjunc aligners using JACUSA2
jacusa2
rnaseq
subjunc
star
rsubread
updated 2 days ago by
Mark
★ 1.7k • written 4 days ago by
thomas.heigl.ibk
• 0
2
votes
1
reply
243
views
RNA-Seq paired-end datasets
paired-end
RNA-seq
Bioinformatic
updated 4 days ago by
Matthias Zepper
5.1k • written 4 days ago by
AIMAR
▴ 50
0
votes
0
replies
218
views
News:
Course: Introduction to CRISPR
GenomeEditing
CRISPR
Evolution
5 days ago by
Physalia-courses
★ 2.6k
3
votes
3
replies
577
views
Best strategy for comparing cell-type composition between disease and healthy groups in single-cell RNA-seq data
Single_cell
updated 3 days ago by
ATpoint
88k • written 5 days ago by
城玮
• 0
1
vote
4
replies
385
views
RNA-seq assigned alignments
paired-end
STAR
RNA-seq
mapping
single-end
updated 4 days ago by
jaro.slamecka
▴ 270 • written 5 days ago by
Manko47
▴ 10
0
votes
1
reply
296
views
Obtain Common SNPS for Mouse Genome
mouse
SNPs
updated 5 days ago by
GenoMax
152k • written 5 days ago by
joe_genome
▴ 60
1
vote
3
replies
462
views
How do you clean GEO metadata before downstream analysis?
geo
updated 5 days ago by
yura.grabovska
▴ 800 • written 7 days ago by
Alba
• 0
0
votes
1
reply
297
views
Seeking high-quality curated metabolic models with time-resolved flux data for sonification project
metabolism
sonification
networks
updated 5 days ago by
Mensur Dlakic
★ 29k • written 6 days ago by
omereilam1
• 0
0
votes
0
replies
235
views
Standard way to represent represent indel (insertion or deletion) alleles within a haplotype
haplotypes
indels
haploview
6 days ago by
Mwangana
• 0
1
vote
1
reply
378
views
lovis4u Configuration
graph
lovis4u
genomics
updated 6 days ago by
GenoMax
152k • written 7 days ago by
NIkita
• 0
1
vote
1
reply
395
views
Tool:
Tool to clean GEO metadata
GEO
metadata
updated 5 days ago by
yura.grabovska
▴ 800 • written 7 days ago by
Alba
• 0
1
vote
2
replies
658
views
WGBS Analysis with 1 control sample and 6 disease samples
one
WGBS
sample
updated 6 days ago by
ATpoint
88k • written 18 days ago by
Cassandra
▴ 10
0
votes
2
replies
665
views
How to extract gene list from GEO dataset in R
coding
beginner
question
r
updated 7 days ago by
Shohel
• 0 • written 17 days ago by
decorus
• 0
2
votes
6
replies
658
views
RNA seq strandedness (reverse-strand)
seq
reverse-stranded
strand
RNA
5 days ago by
ZuelTech
• 0
0
votes
0
replies
294
views
TFs and enrichment analysis for TRAP sequencing
TRAP
8 days ago by
Genesis
• 0
121,580 results • Page
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Comment: High ref mismatch rate after liftOver from 23andme hg19 to hg38
A: diferent contigs chrM
Comment: Best strategy for comparing cell-type composition between disease and healthy gr
Comment: Exome data analysis computing resources requirement query
Comment: Exome data analysis computing resources requirement query
Answer: RNA-seq DE analysis: repeated measures design (options available, mixed models?)
Answer: Kraken2/bracken with 16S sequencing downstream analysis
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Answer: Annotation comparison files on NCBI of gene assembly
by
GenoMax
152k
> May I ask if there exist a documentation that explains this further? There is a file that describes the specific categories: https://ftp…
Answer: Kraken2/bracken with 16S sequencing downstream analysis
by
andres.firrincieli
3.9k
That paper (https://www.nature.com/articles/s41598-023-40799-x) conflates a taxonomic classifier (Kraken2) with a broader analysis platform…
Comment: inferCNV on Visium 10X: lower residual expression in tumoral spots (heatmap inte
by
GenoMax
152k
Attaching a screenshot for ready reference. Full size PDF linked above. Posted as an issue to InferCNV already: https://github.com/broadin…
Comment: Exome data analysis computing resources requirement query
by
GenoMax
152k
> local SSDs are cheap and very fast And keep in mind that they will wear out, especially if you are writing TB's of data regularly.
Comment: Strandedness issues in STAR quantification
by
GenoMax
152k
Looks like you have already opened an issue on STAR repo: https://github.com/alexdobin/STAR/issues/2650 That was going to be the next sugg…
Comment: How to Remove Phasing Information from a VCF File?
by
Mwangana
• 0
How can I use bcftools +setGT to get genotypes without phase e.g. "|" or "/"? For example, instead of 1|1 or 1/1, I would like to get 11
Answer: Two config files for one Snakemake file
by
Bastien Hervé
6.4k
That is exactly what Snakemake is for. You could create a `sample` variable at the top of a `config.yaml` file and loop over these samples…
Answer: Exome data analysis computing resources requirement query
by
colindaven
7.7k
What others have said, but also - whats your approx budget? - Maybe if low budget you can get a long way by using local SSDs on works…
Comment: Strandedness issues in STAR quantification
by
marek.gierlinski
• 0
I tried STAR counting with an index created without GTF. The results are identical to those based on index created with GTF. Every single c…
Comment: TCGA MSI status
by
GenoMax
152k
You can get some data from GDC portal: https://portal.gdc.cancer.gov/analysis_page?app= and https://portal.gdc.cancer.gov/analysis_page?app…
Comment: TCGA MSI status
by
Muhammad Rohan Ali
• 0
Can I get TCGA somatic mutation vcf files or maf files for MSI - H, MSI-L - MSS samples? I mean open access without any special access…
Comment: feature_ref files in upstream analyses related to Perturb-seq
by
GenoMax
152k
What kind of technology is this data from? If it is Parse tech data then you may need to use their pipeline that you can get from here: htt…
Comment: Summing the counts of duplicated genes in RNA seq data?
by
GenoMax
152k
> duplicated genes What is the origin of those genes. Are they entries with same name in GTF but different chromosomal locations (e.g. cop…
Comment: Plotting of genes using tpm matrix
by
i.sudbery
21k
Yes, the y-axis is reasonable. ALthough the test for differences should come from DESeq, rather than from a wilcox in this case. With four …
Comment: [VG sim, surject, giraffe] How to remove a sample from GFA/GBZ graph
by
saruman
• 0
Done. Thank you.
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