I would like to do the GSEA between two different clusters. I have no idea how to select the DEG for GSEA. I tried to use FindMarkers function(markers = FindMarkers(object = cluster, ident.1 = 0, only.pos = FALSE, verbose = T, logfc.threshold = 0, min.pct = 0,test.use = "wilcox")) and would like to get DEG of the whole gene lists. However, several hundred genes miss finally (from 19470-19074), and the pval of the top genes raking by logFC is 0. Could you please help me explaining this phenomenon? Do you have any recommendations for single cells GSEA. Thanks in advance!