Question: Identifying appearance or disappearance of cell populations between WT and Mut single cell RNA-Seq data
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gravatar for Apoorva
8 months ago by
Apoorva270
United States
Apoorva270 wrote:

Hi everyone,

I've done single cell RNA-seq data analysis using Seurat. Now if I have 2 samples, a wild type and a mutant and I want to find out if any new cell populations appear or any cell population disappears in the mutant, how do i test for it ?

I looked at this tutorial and it explains integration and finding differences in gene expression and identifying DEG across conditions, but not to check if there are any new populations appearing. What is the best way to do that ?

seurat single cell rna-seq • 327 views
ADD COMMENTlink modified 8 months ago by igor12k • written 8 months ago by Apoorva270
0
gravatar for igor
8 months ago by
igor12k
United States
igor12k wrote:

You identify your clusters. Then you check the proportion of each sample within your clusters. It' unlikely that you will have a cluster completely unique to one sample. However, you may have a cluster that is, for example, 20% of one sample, but only 1% of the other sample. Thus, that population is enriched in the first sample.

ADD COMMENTlink written 8 months ago by igor12k
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