Is it appropriate to apply quantile normalization on RNA-Seq before computing the Pearson correlation? Specifically, RNA-Seq data displays a dense tail distribution, and Person's correlation (
cor function in R) assumes a normal distribution. I tried this in R using the
normalize.quantiles function discussed in the lecture, and my results make a lot of sense, but I want to make sure that this is an appropriate transformation before accepting these results.
Thank you for your help!