Assemble A1 and A2 reads using Trinity before running blastx on them

Question

[Kash]

I have two fast file A1.fa and A2.fa for a fungi genome. I want to run blastx on these nucleotide sequences.

My question is do I need to use Trinity to assemble A1.fa and A2.fa files in to a single Trinity.fa file and then run blastx on Trinity.fa file?

[Ram]

Why are you considering Trinity? What do you think Trinity does? What do your FASTA files contain? Please describe your premise in as much detail as you can - this question gives us nothing to work with.