Hi community!!! I am analysing already submitted (in SRA) metagenome sequence data by MOTHUR software. here is some confusions
I am seeing in SOP the
cut offvalue for
dist.seqsstep is set to
0.03. In some other protocol I've also seen to set as
0.2. So, I'm confused, what value should I actually use for my study.
Should I use the same value for cutoff in
cluster(column=, count=, cutoff=)step also?
Can anyone please help me?