Question: Cut off for dist.seqs step in MOTHUR
gravatar for dpc
3 months ago by
dpc140 wrote:

Hi community!!! I am analysing already submitted (in SRA) metagenome sequence data by MOTHUR software. here is some confusions

  1. I am seeing in SOP the cut off value for dist.seqs step is set to 0.03. In some other protocol I've also seen to set as 0.2. So, I'm confused, what value should I actually use for my study.

  2. Should I use the same value for cutoff in cluster(column=, count=, cutoff=) step also?

Can anyone please help me?

metagenomics 16s mothur • 105 views
ADD COMMENTlink written 3 months ago by dpc140
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