Entering edit mode
3.8 years ago
nattzy94
▴
50
Hi,
I am doing differential expression using DESeq2 and trying to learn more about independent filtering.
I used the function metadata(res)$filterThreshold
and got the following numbers but don't quite understand them.
> 66.02757%
> 6.589014
I understand DESeq2 filters out genes of a mean normalised count below the threshold. In this case, does this mean that DESeq2 has filtered out genes with a mean count of 6.589? And based on this counts threshold, 66% of genes were filtered out of the analysis?
If so, is the mean count threshold abnormally low? Or is this quite normal?
Thank you!