I have medusa-scaffold data, which contain 160 scaffolds and some NNN bridges. The file is in fasta format, I need to remove all scaffolds less than 200, and vectors possible contamination, this why I am trying to use NCBI Vecscreen. VecScreen However, as a beginner, I still do not know where can I find the option of removing these <200 bp, and how to upload my fasta data, since I have no accession number yet.
Please let me know if you are familiar with such a tool. Many thanks!