Annotation for miRNA-seq data analysis
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2.0 years ago
Arindam Ghosh ▴ 450

For RNA-seq (mRNA/lncRNA) data analysis, we can use reference genome and annotation from Ensembl (GRCh38). Can the same annotation file be used for miRNA-seq data analysis? Even though miRNA annotations are present in Ensembl, most works that I have come across use annotations from other databases like miRBase. Is there any specific reason for this that I am missing out? Ensembl genomes/annotations are convenient to use as they contain all gene biotypes in a single place.

mirna hisat ensembl RNA-Seq Assembly • 787 views
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2.0 years ago

Ensembl imports miRNAs from miRBase: https://www.ensembl.org/info/genome/genebuild/ncrna.html

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Exactly! This is why I was curious to know why most studies separately use miRBase annotations for miRNA even though they are already in the Ensembl reference genome. So I believe there should not be any harm in using the Ensembl GTF file with featureCounts for estimating miRNA expression.

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