Complete novice here so please excuse ignorance and let me know if I need to provide more information.
I'm following the biostars handbook example for RNAseq data (https://www.biostarhandbook.com/rnaseq-align-data.html) and am getting the following error in my terminal:
Error in .local(object, ...) : NAs found in size factors. Have you called already 'estimateSizeFactors'? Calls: estimateDispersions -> estimateDispersions -> .local Execution halted
when running the command
cat simple_counts.txt | Rscript deseq1.r 3x3 > results.txt
resulting in an empty results.txt file. Thanks!