I wish to find out actively expressing genes from the llumina HumanHT-12 v4 Expression BeadChip. I also generated the background corrected and normalized signal intensities for every gene in each sample using the normexp function in limma. Is it possible to draw any conclusions related to absolute gene expression from the normalized signal intensities? Is it appropriate to take average of the signal intensities across samples and based on an arbitrary cut-off term some genes to be actively expressing in all samples?
I'm a novice in microarray data analysis. Please help.