Muscle Alignments On Amazon Ec2 Reset/Crash

Question

[User 7351]

I am conducting some rather large alignments on the cloud and each time am getting a server reset/crash on my Amazon EC2 extra large computing instance. What is going on here? How much RAM do I need to do these alignments?

[Andreas]

How many sequences are you trying to align and how long are they? Do you have some kind of log-file you could post?

[Andreas]

Shameless plug: if you're aligning protein sequences, try Clustal Omega. It uses much less memory during the pairwise alignment stage than others methods

Answer 1

[Istvan Albert]

15GB is not that large amount when it comes to memory - try the High-Memory Quadruple Extra Large Instance (what a silly name ;-) that gives you 64GB.

[Jeremy Leipzig]

they need to get their act together. my grandmother has 64GB. ok well, not quite.

[Istvan Albert]

I think the issue is rather that it is not that easy to build a system that can offer 64GB in a cloud settings. Hard disk and CPUs are a lot easier to multiply.