Question: Muscle Alignments On Amazon Ec2 Reset/Crash
2
gravatar for User 7351
7.5 years ago by
User 735140
User 735140 wrote:

I am conducting some rather large alignments on the cloud and each time am getting a server reset/crash on my Amazon EC2 extra large computing instance. What is going on here? How much RAM do I need to do these alignments?

cloud • 1.2k views
ADD COMMENTlink written 7.5 years ago by User 735140

How many sequences are you trying to align and how long are they? Do you have some kind of log-file you could post?

ADD REPLYlink written 7.5 years ago by Andreas2.4k

Shameless plug: if you're aligning protein sequences, try Clustal Omega. It uses much less memory during the pairwise alignment stage than others methods

ADD REPLYlink written 7.5 years ago by Andreas2.4k
1
gravatar for Istvan Albert
7.5 years ago by
Istvan Albert ♦♦ 81k
University Park, USA
Istvan Albert ♦♦ 81k wrote:

15GB is not that large amount when it comes to memory - try the High-Memory Quadruple Extra Large Instance (what a silly name ;-) that gives you 64GB.

ADD COMMENTlink written 7.5 years ago by Istvan Albert ♦♦ 81k

they need to get their act together. my grandmother has 64GB. ok well, not quite.

ADD REPLYlink written 7.5 years ago by Jeremy Leipzig18k

I think the issue is rather that it is not that easy to build a system that can offer 64GB in a cloud settings. Hard disk and CPUs are a lot easier to multiply.

ADD REPLYlink written 7.5 years ago by Istvan Albert ♦♦ 81k
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