Spatial Transcriptomics : Compute expression correlation between two adjacent slides (technical replicates)
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3.8 years ago

Hi,

We sequenced two adjacent slides (10X) from the same sample in order to check reproducibility. We would like to calculate expression correlation between both slides.

Data are stored in Seurat object.

As you can see both tissues are not perfectly aligned on the 10X slide.

Is there a way to compute correlation between each gene and spot by correcting for this offset of positions between both technical replicates ?

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spatial transcriptomics • 636 views
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