How to obtain the chromosomal location for a list of 8M SNPs?
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11 months ago
aroso491 • 0

Hello, I have a list of 8M SNPs from a meta-GWAS which does not include the chromosome ID and chromosome location in bp. I thought I could do this using the grch37.ensembl.org mart of bioMart (I have never done this before and I am still learning), but I have read in other posts that there is a 500 line limit.

My list of rsIDs is of about 8.5M and I have read in other posts that there are other ways to do this like Parse the VCF, Use the APIs or Use the VEP.

The thing is that I do not know how to do any of these and I have not managed to find any sort of tutorial or anything to learn to do that. Do any of you have any sort of indication about where can I learn to retrieve the chromosomal location and ID for my list, please?

Thanks in advance.

VCF SNP R ensembl bioMart • 328 views
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An alternate way would be to use galaxy https://usegalaxy.org/

  • In the left tools search bar searching for "UCSC"
  • "UCSC Main table browser" click redirects to ucsc table browser
  • Here group "variation" and dbSNP table can be selected
  • Then in "get output" -> "send query to galaxy"
  • The job might take a while but the the bed of dbSNP with rsids will be uploaded to galaxy
  • Then one can upload the file with rsids and use "join two datasets side by side" tool to get the join of the dnsnp location of the uploaded SNPs
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Thank you so much! This sounds exactly like what I needed. I am giving it a try right now, will let you know if it worked. :)

Edit: It worked just fine, although it's only been able to retrieve 3.5 million out of my 8 million file. I assume this is normal as some of the SNPs might just not be in the database or identified yet, but I was wondering if there'd be a way to double check this.

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