Entering edit mode
3.8 years ago
prathyushareddy87
▴
10
Hi, I have an experiment that was run using AmpliSeq Thermo Fisher Sequencer. The output was Reads Per Million. I am not very much familiar with AmpliSeq technology. I am not sure if I need apply any sort of normalization technique here. Currently I did log2(RPM+1) transformation to make them log2 transformed. Is the methodology correct? Or am I missing anything here. The goal is to run the differential gene expression analysis and counts are not available and I don't have access to raw data. Any help is much appreciated.
Best, Prathyusha
