How to make consensus sequences for a list?

Question

[adrian18_07]

I have a sequence list, it looks like this:

[['CATGCCCGACAGAGCGACCCGCGAACACGTTACAAACACTACGCGGGGTGGCCCCGGCTGCCTCGCGCGGAGGTGCTGCGGCTGAGTGCGCAAACTAGCTGCGCGCACGCTGTCCGTGCCACCTCCACTAACAGAACCCCGGCGCGGACTGCGCCAAGGAATAAAAAACGAATGAGAGCGAGCGCGCCCCCCTCGCCCCGGAGACGGTGCGCGATGGTGTGTGCCTCGCTGTCCATTGATAAACTAAACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCGATGAARAACGTAGCGAAATGCGATACTTGGTGTGAATTGCARAATCCCGTGAATCATCGAGTCTTTGAACGCAAGTTGCGCCCGAAGCCTTCTGGCCGAGGGCACGTCTGCCTGGGTGTCACGCAACGTCGCCGCCAACCCCACCCCTAGGGGCGGGAAGTTGGGGGCGGACTCTGGCCTCCCGTGCGCCTCGGCGCGCGGATGGCCTAAATTTCAGCTCCTGGCGAGGATCGCCACGACAAGCGGTGGTTTTTTGAACTAAGGACCTCGGGTGTTGTCGTGCGGCCTCCCGGAGGGAACGGACCCTGTGCGCTCGCGCACCATCCTATCGAGACCCCAGGTCAGTCGG', 'CATGCCCGACAGAGCGACCCGCGAACACGTTACAAACACTACGCGGGGTGGCCCCGGCTGCCTCGCGCGGAGGTGCTGCGGCTGAGTGCGCAAACTAGCTGCGCGCACGCTGTCCGTGCCACCTCCACTAACAGAACCCCGGCGCGGACTGCGCCAAGGAATAAAAAACGAATGAGAGCGAGCGCGCCCCCCTCGCCCCGGAGACGGTGCGCGATGGTGTGTGCCTCGCTGTCCATTGATAAACTAAACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAATCATCGAGTCTTTGAACGCAAGTTGCGCCCGAAGCCTTCTGGCCGAGGGCACGTCTGCCTGGGTGTCACGCAACGTCGCCGCCAACCCCACCCCTAGGGGCGGGAAGTTGGGGGCGGACTCTGGCCTCCCGTGCGCCTCGGCGCGCGGATGGCCTAAATTTCAGCTCCTGGCGAGGATCGCCACGACAAGCGGTGGTTTTTTGAACTAAGGACCTCGGGTGTTGTCGTGCGGCCTCCCGGAGGGAACGGACCCTGTGCGCTCGCGCACCATCCTATCGAGACCCCAGGTCAGTYAG'], ['CATGCCCGACAGAGCGACCCGCGAACACGTTACAAACACTACGCGGGGTGGCCCCGGCTGCCTCGCGCGGAGGTGCTGCGGCTGAGTGCGCAAACTAGCTGCGCGCACGCTGTCCGTGCCACCTCCACTAACAGAACCCCGGCGCGGACTGCGCCAAGGAATAAAAAACGAATGAGAGCGAGCGCGCCCCCCTCGCCCCGGAGACGGTGCGCGATGGTGTGTGCCTCGCTGTCCATTGATAAACTAAACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCGATGAARAACGTAGCGAAATGCGATACTTGGTGTGAATTGCARAATCCCGTGAATCATCGAGTCTTTGAACGCAAGTTGCGCCCGAAGCCTTCTGGCCGAGGGCACGTCTGCCTGGGTGTCACGCAACGTCGCCGCCAACCCCACCCCTAGGGGCGGGAAGTTGGGGGCGGACTCTGGCCTCCCGTGCGCCTCGGCGCGCGRATGGCCTAAWTTTCAGCTCCTGGCGAGGATCGCCACGACAAGCGGTGGTTTTTTGAACTAAGGACCTCGGGTGTTGTCGTGCGGCCTCCCGGAGGGAACGGACCCTGTGCGCTCGCGCACCATCCTATCGAGACCCCA', 'CATGCCCGACAGAGCGACCCGCGAACACGTTACAAACACTACGCGGGGTGGCCCCGGCTGCCTCGCGCGGAGGTGCTGCGGCTGAGTGCGCAAACTAGCTGCGCGCACGCTGTCCGTGCCACCTCCACTAACAGAACCCCGGCGCGGAYTGCGCCAAGGAATAAAAAACGAATGAGAGCGAGCGCGCCCCCCTCGCCCCGGAGACGGTGCGCGATGGTGTGTGCCTCGCTGTCCATTGATAAACTAAACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCGAAATGCGATACTTGGTGTGAATTGCAGAATCCCGTGAATCATCGAGTCTTTGAACGCAAGTTGCGCCCGAAGCCTTCTGGCCGAGGGCACGTCTGCCTGGGTGTCACGCAACGTCGCCGCCAACCCCACCCCTAGGGGCGGGAAGTTGGGGGCGGACTCTGGCCTCCCGTGCGCCTCGGCGCGCGGATGGCCTAAATTTCAGCTCCTGGCGAGGATCGCCACGACAAGCGGTGGTTTTTTGAACTAAGGACCTCGGGTGTTGTCGTGCGGCCTCCCGGAGGGAACGGACCCTGTGCGCTCGCGCACCATCCTATCGAGACCCCA']]

I would like to receive consensus sequences in turn for each sub-list. I tried this way:

from Bio.Align import AlignInfo
for z, _ in enumerate(pp):
    summary_align = AlignInfo.SummaryInfo(pp[z])
    consensus = summary_align.dumb_consensus()
    print(consensus)

But I get the error:

 File "C:\...", line 93, in <module>
consensus = summary_align.dumb_consensus()

 File "C:\...", line 77, in dumb_consensus
con_len = self.alignment.get_alignment_length()

AttributeError: 'list' object has no attribute 'get_alignment_length'

Thanks for any answer.

[Joe]

How do you propose to define a consensus between 2 sequences? There is no way to decide which residue should be chosen.

Please also go back and accept answers on your previous questions if they have resolved the problem for you, e.g.:

• Removal of primers from matched sequences
• How to index SeqRecord objects in the list?

[Joe]

In addition to my other comment, its not clear what pp is in this case,or why you need to use enumerate to access elements of it instead of just iterating the object itself?

I suspect you aren't passing the object you think you are which is why its failing to find the .get_alignment_length() method.