Question: Size of the demultiplexed fastq files
0
gravatar for Lucas
22 days ago by
Lucas0
Lucas0 wrote:

Hi

I've demultiplexed a fastq file into the individual samples, but it seems there is something wrong, the size of the individual samples is various, and this difference sometimes is really high, for example about 1 GB. Is it normal?

Almost all the samples volume is the same, for example 1.4, 1.5 or 1.6 GB but some files have higher volume about 2.1 GB.

Thanks,

Lucas

ADD COMMENTlink modified 22 days ago by genomax87k • written 22 days ago by Lucas0
2
gravatar for genomax
22 days ago by
genomax87k
United States
genomax87k wrote:

It is normal to have data files of various sizes. This could be due to imbalance of the sample concentration in original pool and/or quality of the individual libraries (e.g. if libraries have short inserts so adapters get trimmed from sequence when you have sequence read-through).

ADD COMMENTlink modified 22 days ago • written 22 days ago by genomax87k

Thank you very much.

ADD REPLYlink written 22 days ago by Lucas0
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