What I have is six RNA-Seq datasets from three developmental stages in two conditions (control and treatment), without biological and technical replicates. (I did all the following in usegalaxy.eu) Each dataset was mapped to reference genes using tophat. htseq-count was used to count aligned reads. I tried to use DESeq2 for differential expression analysis but it works only when I have replicates. Is there any way/tool to do DE without biological and technical replicates? suggestions considering Galaxy friendly tools are preferred. Thank you so much
Note: The data belongs to a friend who said that they have no replicates because of budget shortages.