Hi I got hundreds of warning "reached iteration limit" when I run
Sproject <- SCTransform(Sproject, vars.to.regress = c("percent.mt", "percent.rps", "percent.rpl", "percent.rrna", "nCount_RNA", "nFeature_RNA"), verbose = FALSE, return.only.var.genes = FALSE)
and the warning not stop until I stop running the code. Does anyone face the same issue? Is there any solution?
What is alternative function to replace SCTransform?