I ran a demultiplexing set on 12 samples with the SMRTtools (PacBio). For that, I used 12 different barcodes in FASTA format.
All of the 12 barcodes are found, but there is an extra barcode found (different pair in the code below). I guess it disturbs the demultiplexing analysis.
With same pair : 647416 (100%) With different pair : 1992 (0%) Coefficient of correlation : 136.93%
Why there is an extra barcode found, while in my other demultiplexing analysis, only the barcodes used during the library prep are found?