Gene Ontology result interpretation produced by AGRIGO

Question

[hirahameed222]

Hello, I have performed RNA-seq analysis for differential gene expression using hisat, stringtie and ballgown. i have fetched the gene names and performed an Ontology by passing geen names as a list on online tool names as AGRIGO and they generated the result in figure form. i cant understand and read the output generated. neither i am able to write it enough as a figure legend. can someone please help me how can i write about the ontology in precise and understandable way. I am new to this line and i will be really thanlful for the help.

! GeneOntolgy result produced by AGRIGO showing cellular analysis

[antonioggsousa]

Hi,

Did you check the manual: http://bioinfo.cau.edu.cn/agriGO/manual.php

The graphical result is a GO hieratical image containing all statistically significant terms. These nodes in the image are classified into ten levels which are associated with corresponding specific colors. The smaller of the term's adjusted p-value, the more significant statistically, and the node's color is darker and redder (Note: adjusted p-value here means that the value of the multiple-test adjusted p-value). Inside the box of the significant terms, the information includes: GO term, adjusted p-value, GO description, item number mapping the GO in the query list and background, and total number of query list and background. But when those term whose adjusted p-value is higher than the cutoff set by the user, only GO information will be given in the box. To better understand the graphical result, investigation of the annotation diagram is suggested.

António