Can we compare bulk RNAseq samples and single cell rnaseq clusters?
1
0
Entering edit mode
9 months ago

Hi folks,

I have 3 Bulk RNAseq samples from immune cells (Naive, Population1 and Population2), similarly I have two other immune populations from Single Cell RNAseq samples.

I am interested in comparing 3 Bulk RNAseq and 2 single cell RNA seq. Any thoughts, papers and suggestions are welcomed.

Thanks in advance.

RNA-Seq Bulk Single cell Immune cells • 900 views
ADD COMMENT
0
Entering edit mode

What do you mean by "comparing" ?

ADD REPLY
0
Entering edit mode
8 months ago

I don't know - perhaps define clusters in the scRNA-seq data, get the genes that are highly expressed in these clusters, and then check for the expression of these in the bulk data.

Similar to the above, you could also do a rudimentary de-convolution by defining a signature for each identified scRNA-seq cluster, and then use these in the bulk data in order to infer cell-types [in the bulk data].

Kevin

ADD COMMENT

Login before adding your answer.

Traffic: 2743 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6