Question: Partial Mapping Issues
0
gravatar for eoz222
17 days ago by
eoz2220
eoz2220 wrote:

Hi all,

I was wondering what might cause this partial mapping pattern.enter image description here

alignment assembly • 85 views
ADD COMMENTlink modified 17 days ago • written 17 days ago by eoz2220
1
gravatar for swbarnes2
17 days ago by
swbarnes28.2k
United States
swbarnes28.2k wrote:

I think you've got some kind of adapter on the end of every read, so the half that is sample maps, and the half that is adapter is all mismatched. I'd eyeball your reads, see if you can spot a common sequence on all of them.

ADD COMMENTlink written 17 days ago by swbarnes28.2k
0
gravatar for eoz222
17 days ago by
eoz2220
eoz2220 wrote:

Thanks for the reply. So I downloaded this data from NCBi to do some data mining and noticed that it is a RAD-seq dataset. I am not sure whether this pattern is caused by the method or something else is going on. PS: I am not really familiar with RAD-seq method.

ADD COMMENTlink written 17 days ago by eoz2220
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