Question: Barnyard analysis advice
0
gravatar for cf556
16 days ago by
cf55610
cf55610 wrote:

I have a scRNA dataset with both human and mouse cells. Due to the upstream experiment, there is no way to physically separate the cells by species, nor is there a detectable fluorophore or another modification that could be used to isolate cells from one species or another.

I am wondering if there is a recommended way to identify the species of origin on a cell by cell basis? Would a list of genes that have no ensemble translations across the genomes be a good place to start? Would counts mapped to mouse vs human cells be considered meaningful? Is there an established pipeline for this sort of thing?

Thanks!

rna-seq scrna R barnyard • 68 views
ADD COMMENTlink written 16 days ago by cf55610
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 902 users visited in the last hour