Question: over-representation of sequences that map to "Drosophila melanogaster signal recognition particle
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gravatar for  DataFanatic
15 days ago by
DataFanatic240
DataFanatic240 wrote:

I found over-representation(count:114784, 1.65%, No Hit) of sequences that map to "Drosophila melanogaster signal recognition particle" but with my current knowledge, I am still unsure whether I should remove these reads from my TRAP-Seq data.

"signal recognition particle (SRP) controls the transport of secretory proteins into and across lipid bilayers"

https://blast.ncbi.nlm.nih.gov/Blast.cgi#alnHdr_1834199030

ADD COMMENTlink written 15 days ago by DataFanatic240

Why do you want to remove some sequences? Without more details on the experiment and how the data was processed, it's impossible to answer.

ADD REPLYlink written 15 days ago by Jean-Karim Heriche23k

The data is intact, I just ran FastQC on the fastqc files. I said I was unsure not that I want to remove the sequences. I was hoping someone could explain if this is a typical artifact of Ribo-Seq data (others have posted a similar question with no answers yet). Thanks for your comment though.

ADD REPLYlink written 14 days ago by DataFanatic240
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