Question: single cell ATAC-seq cicero gene activities in Seurat
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gravatar for el24
13 days ago by
el2410
USA
el2410 wrote:

Hi everyone!

I am loading my single-cell ATACseq data using cicero, and then I create gene activity scores according to the tutorial. My goal is to use this cicero gene activity score object in Seurat. Based on Seurat, I can use cicero object in their pipeline as they mentioned that "Seurat's method is compatible with any method that returns a gene by cell-matrix (e.g. Cicero)"

My problem is that I have tried this cicero object as an input for several functions of Seurat's tutorial, but it gives me errors all the time. (I tried it in CreateGeneActivityMatrix(), CreateSeuratObject(), and etc.) For example when I run the following:

activity.matrix <- CreateGeneActivityMatrix(peak.matrix = cicero_gene_activities, annotation.file ="data/Homo_sapiens.GRCh38.100.gtf", seq.levels = c(1:22, "X", "Y"), upstream = 2000, verbose = TRUE)

Error in .get_data_frame_col_as_numeric(df, granges_cols[["start"]]) : 
some values in the "start" column cannot be turned into numeric values

Then, I ignored the first few functions that throw an error and tried going through the rest of the tutorial, but then I faced an error here:

pbmc.atac <- RunLSI(pbmc.atac, n = 50, scale.max = NULL)
     Error in as(object = data, Class = "dgCMatrix") : 
     no method or default for coercing "numeric" to "dgCMatrix"

I am very new to Seurat so I couldn't figure the errors out by searching. I would appreciate any comments on how to proceed forward with Seurat using my cicero object.

Thanks in advance!

ADD COMMENTlink written 13 days ago by el2410
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