I wanted to obtain TCGA vs GTex differentially expressed genes using either DESEQ2 or EdgeR; however, I cannot use Amazon and Galaxy failed to do the job, and the amount of RAM on my laptop didnt allow the computation. I thought using non-bayesian techniques might solve my problem. Should I give it a try? What Nonbayesian R packages are commonly used? On the other hand, Is it possible to filter genes to keep only 20% or so top variable ones without prior estimates? I am starting my analysis with normalized TCGA+GTEx count data. Thanks in advance.