Why the "Estimated Number of Cells" and when I do dim(seurat_object) have different number of cells?
10X defines "Estimated Number of Cells" as "The number of barcodes associated with cell-containing partitions, estimated from the barcode UMI count distribution."
Eg:My sample in 10X summary report says "Estimated Number of Cells" is 8,388. However when I load the object in Seurat and do dim(seurat_object), I get 53847 cells.
What am I missing?