Hello all. I'm REALLY trying to get DEG analysis done for my PI with essentially no experience and no guidance. I'm using Galaxy and attempting to assemble the fastq files received via sequencing and am getting very strange results. FastQC shows very odd GC%:
The major thing I notice is the same small sequence following by a + and then the unique sequence directly after. I do not see this in other sets of files that seem to be working very well. I've searched google with every combination of search terms that I can think of to understand what this is and how to solve it. I will be GREATLY appreciative to anyone who can help me, as I have a lot of pressure to get this done without any help. Thank you very much.