Bowtie alignment reporting
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3.7 years ago

What is the difference between bowtie alignment reporting options

I) -k 1 and II) -a --best --strata

Both reports best one alignment ? If not please clarify.

option I)
if -k is 1, it will report up to 1 good alignments per read.

option II)
-a option tells bowtie to report all the alignments.
--best options tells bowtie to output all the alignments but in an increasing order of mismatches.
--strata will only report the best alignment for a read.
(source : As per bowtie1 -m --best --strata options )

alignment • 693 views
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