Question: A peak on FastQC graph Sequence Duplication Levels on MNase seq data
0
gravatar for daria.d.kozhevnikova
5 weeks ago by
daria.d.kozhevnikova0 wrote:

Good time to anyone! Here I have the result of MNase seq data analysis using FastQC. The problem is in this peak of duplicated sequences at the score >10. Its not good for data quality, I don't know why such a picture appear. If somebody've faced this problem before I will be very grateful to you for answer!

enter image description here

ADD COMMENTlink modified 5 weeks ago • written 5 weeks ago by daria.d.kozhevnikova0

I am not familiar with MNase seq but it is normal to have a result like this depending on the context of the experiment. Please take a look at this blog post from authors of FastQC for alternate explanations, if this was not expected.

Have a fail in FastQC does not make the data bad. It all depends on type of data you are working with.

ADD REPLYlink written 5 weeks ago by genomax89k
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 732 users visited in the last hour