Question: A peak on FastQC graph Sequence Duplication Levels on MNase seq data
gravatar for daria.d.kozhevnikova
5 weeks ago by
daria.d.kozhevnikova0 wrote:

Good time to anyone! Here I have the result of MNase seq data analysis using FastQC. The problem is in this peak of duplicated sequences at the score >10. Its not good for data quality, I don't know why such a picture appear. If somebody've faced this problem before I will be very grateful to you for answer!

enter image description here

ADD COMMENTlink modified 5 weeks ago • written 5 weeks ago by daria.d.kozhevnikova0

I am not familiar with MNase seq but it is normal to have a result like this depending on the context of the experiment. Please take a look at this blog post from authors of FastQC for alternate explanations, if this was not expected.

Have a fail in FastQC does not make the data bad. It all depends on type of data you are working with.

ADD REPLYlink written 5 weeks ago by genomax89k
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