Hello, I have a gene expression data from two conditions. Its FPKM value from cuffdiff. I picked 3fold up and down-regulated genes to draw a heatmap. I used different R packages and other online tools, but I keep getting a very uniform color.
- I want to have a more color gradient, which represents my expression values.
- I want to list 360 genes on the row label, can I do it? The font size did not work.
- I believe there is a problem with scaling. When I used cluster optimization method to determine the number of clusters, Its gives me 7 as shown below, when I make the heatmap, even though I gave the parameter for clustering to be 7 I still get two clusters. Any advice on this?