Stacks process_radtags question and scripts
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Entering edit mode
15 months ago

How I can modify the quality score to retain more reads in stacks process_radtags? I have paired-end reads from Illumina Next Seq 500.
This is my script: process_radtags -1 MAGICR1_T.fastq.gz -2 MAGICR2_T.fastq.gz -b Barcode2.txt -o /home/mdominguez/demult_MAGIC_b --renz_1 sphI --renz_2 sau3AI -E phred33 --barcode_dist_2 3 -q -r --disable_rad_check --inline_inline -i gzfastq -t 66

Best regards,

Matias

snp next-gen • 498 views
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Entering edit mode
6 weeks ago
strive • 0

hello,how do you add barcode information,please? the company told me is that barcode has been removed, so there is no barcode in the data. Now I am very confused about how to add?

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