convert SRA to fastq
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1
Entering edit mode
14 months ago

Hi everyone recently I download some SRA files (12 files) using this command from NCBI:

prefetch ERR4172779

all of SRA file were dowloaded properly but when I try to convert them to fastq files using this command :

fastq-dump --split-3  ERR4172779

, it shows this:

2020-08-12T11:42:02 fastq-dump.2.9.4 err: file invalid while opening directory within file system module - failed to resolve column 'READ' idx '1'

2020-08-12T11:42:02 fastq-dump.2.9.4 err: file invalid while opening directory within file system module - failed to resolve column 'READ' idx '1'

2020-08-12T11:42:02 fastq-dump.2.9.4 err: file invalid while opening directory within file system module - failed ERR4172779.sra

==================================================

An error occurred during processing.
A report was generated into the file '/home/mohammad/ncbi_error_report.xml'.
If the problem persists, you may consider sending the file
to 'sra-tools@ncbi.nlm.nih.gov' for assistance.

what's the problem??

SRA toolkit FASTQ fastq-dump • 619 views
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1
Entering edit mode

Hello mo.ghasemi717,

Please use the formatting bar (especially the code option) to present your post better. I've done it for you this time.
code_formatting

Thank you!

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1
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a tool I found very helpful for retrieving data is the following:

https://sra-explorer.info/

it says SRA, but you can also get download links for ENA (and I second dariober post below to use ENA rahter than SRA for this)

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3
Entering edit mode
14 months ago

Looking at the ENA archive here, it seems fastq files are not available for this accession. However, they provide BAM files.

(As a side note, in my opinion is better to work with ENA than SRA, see also this answer of mine at https://bioinformatics.stackexchange.com/a/14121/339)

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thank you dear dariober for your explanation

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