differential expression from sc RNA seq
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21 months ago
re_raz ▴ 20

Hello I hope everyone staying safe and healthy I have question which might be stupid question. This because I am new to bioinformatic and single cell RNA seq analysis. I hope someone help me. The data set consists of 12 samples scRNA-seq and I want to get differential expression to compare between samples. I merged my data, created Seurat Object, and did normalization, scaling and clustering. How I calculate differential expression for my samples?

Thanks a lot

scRNA -seq DE Seurat • 1.5k views
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I'm assuming you want to find differential expression for each cluster among different samples?

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yes, exactly that what I am going to do but I don't know how to start. Now all my samples merged and got clusters for them. I did search but all I found for RNA seq not single cell RNA-seq

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