Hi everyone, I am trying to use RSEM and want to use STAR as my aligner. I'm not sure what argument I am missing/using wrong. But I keep getting this error:
Invalid number of arguments!
The online tutorial is not up to date. In fact, I was able to debug many errors by reading through forums. Anyway, here is the command:
rsem-calculate-expression -p 2 --star-gzipped-read-file --star \ --star-path /path/to/star/executable --paired-end \ /path/to/fastq.gz.1 /path/to/fastq.gz.2 \ STAR_genome_dir sample_name_mouse
Thanks in advance for the help/suggestions!