How take advantage of ERCC spike-ins to improve variant calling
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14 months ago

I have ERCC-spike-ins in my RNA-sequencing data and I do see some base changes in the VCF after running GATK. I assume these are errors as a result of the sequencing process but I am not sure how to correct for them. I haven't done variant calling before so I was wondering if anyone has any ideas?

SNP RNA-Seq indel • 281 views
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